Sample Submission Guideline
We would recommend submitting aliquots of your samples.
NGS Libraries prepared by you
- Submit libraries at 10-20 nM in a volume of at least 10ul.
- Submit index information of your libraries and how your libraries were made.
DNA & RNA For Illumina library preparations
- It is recommended to use a fluorescence-based quantification for your nucleic acid. Nanodrop can be used to measure the ratio of A260/280 and A260/230.
- Dilute your RNA and DNA in nuclease-free water.
- If possible, please check quality of your DNA by high molecule weight on 1% agarose gel or your RNA by checking RNA integrity number on Agelient Bioanalyzer.
- RNA must be treated with DNase I to remove any DNA contamination.
- Please refer the required quantity and quality of nucleic acid in the table;
| Sample type | Conc. (ng/ul) | Minimum vol. (ul) | A260/280 | RNA Integrity Number |
|---|---|---|---|---|
| RNA | 100 | 15 | > 2.0 | >7 |
| DNA | 10 | 15 | > 1.8 |
Single cell library sample guidelines
- Consultation before submitting samples is required.
- Submit cells at 1,000 cells/ul in PBS-BSA with ideal viability of 90%.
- Submit the sample submission form 24-48 hours before your experiment.
- Sample submission deadline time is 1 pm on the day of experiment.
Sanger sequencing samples guidelines
- When samples dropped off by 12 pm, the results will be delivered in one business day except for 48 or more samples.
- Submit your samples in 0.2ml, 8-tube PCR strips.
- Provide primer and template premixed samples according to the guidelines below.
| DS plasmid DNA | PCR product | BAC and Phage Lambda DNA | Single-stranded M13 or Phagemid DNA | |
| Template | 1,000-1500 ng | 100-300bp: 40-50ng 300-500bp: 40-160ng 500bp-1kb: 500ng | 4000 ng | 200-300 ng |
| 4 µM primer | 2 ul | 2 ul | 2 ul | 2 ul |
| Sterile water | X ul | X ul | X ul | X ul |
| Total volume | 14 ul | 14 ul | 14 ul | 14 ul |
- PCR Cleanup: Please be sure samples are cleaned up prior to submission, not just diluted or desalted. Size exclusion columns are recommended. EtOH precipitation is not recommended as this does not remove unbound dNTPs and primer.
- BAC and Phage Lambda DNA: Specify BAC or Lambda when ordering. Only high purity templates should be used to optimize the amount of DNA loaded onto the sequencer.
- Recommended BAC template preparation methods: Alkaline lysis with phenol extraction and isopropanol precipitation or Cesium Chloride Banding
Please complete the sample submission form by clicking the button above. Once the form is reviewed, you’ll be contacted to bring the samples to the UTD Genome Center (BSB 12.610).
ACKNOWLEDGEMENT GUIDELINE
If you used the Genome Center to generate data for your publications, posters, grant proposals, or presentations, please use the below examples for your acknowledgement section.
You may use the following examples for acknowledgment section at the end of manuscript;
- The authors acknowledge the Genome Center at The University of Texas at Dallas for their support during this course of research.
- We thank the Genome Center at The University of Texas at Dallas for the services to support our research.
You may use the following example in the Material and Method section;
_________ was prepared/performed by the Genome Center at The University of Texas at Dallas (Richardson, TX).Please feel free to contact us if you need more detailed information about the services you used for the Material and Method.
If you need more information on acknowledging the core or co-authorship of core members, please utilize the Recommended Guidelines for Authorship on Manuscript published by the Association of Biomedical Resource Facilities (ABRF).
We would love to keep track of our contributions to the science community. Please email us when your work is published or a grant is awarded using the data we helped to generate.