Run samples for the Aria through a cell strainer immediately before bringing them to the core to prevent clogs.
Bring samples and collection tubes in containers with closeable lids on ice (Coleman camp coolers work great). Bring extra sample buffer in case samples need to be diluted.
Collection tubes for the sorter should have high serum concentration (~30%) in cell media (such as RPMI) to compensate for dilution by sheath fluid.
Single-color controls should be performed regularly, especially for sensitive assays. For difficult-to-find populations, FMO (fluorescence minus one) controls can make it easier to remove background noise.
Viability markers will increase the fidelity of cultured populations by excluding dead or dying cells.
When sorting, periodically spin down collection tubes to pellet cells, reducing viability losses. For very long sorts, also refresh ice buckets.
Spectra analyzers can help with panel design to reduce compensation problems. The core will be happy to advise, as well.
