The Agilent GCMS is equipped with a single quadrupole 5975C mass select detector and a 7890A Gas Chromatograph, This system has a pressure setpoint precision to 0.001 psi and high retention time reproducibility. It has an electron impact (EI) ionization source and equipped with a standard automatic 150 vial liquid sampler and headspace sampler. This GCMS is used to identify and quantify small molecular metabolites (50-650 daltons), including TCA intermediates, glycolysis, fatty acids, amino acids, sugars, fatty acids after chemical derivatization to make these compounds volatile enough for gas chromatography. GCMS is also useful for analysis of chemicals that are volatile or be volatile after derivatization. Software resources are MassHunter GCMS operation software, ChemStation data analysis software, MassHunter data analysis includes MassHunter Quant and MassHunter Qual. NIST Spectral Library will be used for compound identification.
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