Laser scanning confocal microscope equipped with a tunable white light laser system, stimulated emission depletion laser for super resolution microscopy, and tunable emission filters for fixed or live sample imaging in the visible range.
Visuals
Capabilities
- Super resolution imaging (~75 nm lateral) via 2D stimulated emission depletion
- Photon arrival time recording and gating via TauSense
- Tunable white light laser system for optimal fluorophore excitation
- Spectrally tunable detectors
- 3D z-stacks, timelapse, multi-position timelapse
- Water pump and lens cap for long timelapse with water-immersion objective lens
Cost
Internal user $33/hour
Manual
Specs
Microscope
- Leica DMi8 fully motorized inverted microscope
- Automated stage with multi-area timelapse and mosaic imaging
- 2D Stimulated emission depletion (STED) module
Scanning Mirrors
- Galvanometer scanner for high-definition imaging (16×16 – 8192×8192, 10-2600 lines/s)
Light Sources
- 405 nm diode laser
- Pulsed white light laser system (up to 8 tunable laser lines, 485 – 685 nm)
- 775 nm pulsed laser for STED super resolution of red/far-red fluorophores
- LED-based transmitted and epifluorescence illumination
Objectives
Air objectives:
- HC PL APO 10x/0,40 CS2 (0.4 NA)
Multi-immersion:
- HC PL APO 20x/0,75 IMM CORR CS2 (0.75 NA)
- HC PL APO 40x/1,1 W CORR CS2 (1.1 NA)
Water-immersion:
- HC PL APO 86x/1,20 W motCORR STED white (1.2 NA)
Oil-immersion
- HC PL APO 100x/1,40 OIL STED white (1.4 NA)
Dichroics
- Conventional dichroic mirrors
Detectors
- 3 HyD detectors (cooled SiPM, 2 HyD S SP, 1 HyD X SP) with tunable emission filters
- Transmitted light detector for brightfield imaging
Computer and Software
- Olympus cellSens Dimension provided for additional post-acquisition data analysis and processing
Posted under Imaging and Histology