Olympus FV3000RS Confocal Laser Scanning Microscope

September 15, 2022

This instrument has been decommissioned.

Laser Scanning Confocal Microscope equipped with 7 laser lines, a fully automated stage for whole tissue stitching, and live cell imaging capability

Location

RL B.717C

Visuals
Credits: Ted Price, Stephanie Shiers; Instrument: FV3000 confocal; Description: Immunohistochemistry of Pyramidal neurons (KV Channel clustering; green), axon initial segments (red), nuclei (blue) in prefrontal cortex of mouse brain.
Credits: Ted Price, Salim Megat; Instrument: FV3000 confocal; Description: Mouse treated with LPS, EGFP-Ribo-Tag (green), IBA1 which stains microglia (red) and NeuN which stains neuronal nuclei (blue).
Capabilities
  • Imaging diverse fluorophores in fixed or live samples
  • Temperature, humidity, CO2 control
  • Spectrally tunable detectors
  • Ultra-fast resonant scanning (reduced photo-damage with live samples)
  • 3D z-stacks, timelapse, multi-position timelapse
  • FRAP/FLIP/Photoactivation, FRET (ratio or acceptor photobleaching)
Cost

Internal user $32/hour

Specs

Microscope

  • Olympus IX83 fully motorized inverted microscope
  • Zero Drift Compensator (IX3-ZDC2) laser-based autofocus
  • Ultrasonic automated stage and multiple area time lapse stage control software, as well as well-plate navigator tool for multi-area, time-lapse, and multi-area mosaic image stitching
  • Tokai hit stage top, live-cell incubation chamber
  • Analog and digital in-out box for synchronization with external devices
  • Facilitates integration of PicoQuant, FCS/FCCS, and FLIM hardware packages

Tandem Scanners

  • Conventional scanner for high-definition imaging
  • Resonant scanner for high-speed imaging (capable of 30 FPS@512×512 and 438 FPS@512×32)

Light Sources

  • All Diode Lasers with 7 Laser Lines–405 nm, 445 nm, 488 nm, 514 nm, 561 nm, 594 nm, and 640 nm
  • LED-based transmitted and epifluorescence illumination

Conventional Fluorescence Filters

  • Dapi
  • Green
  • Red

Objectives

Air objectives:

  • 25X; PLAN APO 1.25X, NA 0.04,WD 5.1 mm
  • UPLSAPO10X2; U PLANS S-APO 10X, NA 0.4, WD 3.1 mm
  • UPLSAPO20X; U PLAN S-APO 20X, NA 0.75, WD 0.65 mm

Silicone oil objectives:

  • UPLSAPO30XS; UPLSAPO N 30X SI OIL, NA 1.05,WD 0.8MM, W/CC (with correction collar)
  • UPLSAPO40XS; UPLSAPO N 40X SI OIL, NA 1.25, WD 0.3MM,W/CC
  • UPLSAPO100XS; UPLSAPO 100X SI OIL, NA 1.35,WD 0.20MM,W/CC

Silicone oil provides better refractive index match to cells and tissue than water or traditional immersion oil for brighter and deeper cell/tissue imaging. Silicone Oil does not evaporate nor degrade permitting very long live cell/tissue time-lapse imaging with ease. Greater working distance than most traditional immersion objectives while still retaining high numerical aperture (30x – 1.05 NA 800 µm WD; 40x – 1.25 NA 300 µm WD; 100x – 1.35 NA 200 µm WD)

Plan Super Apochromat design fully compensates for both planar and chromatic aberrations from the UV to the near infrared region for precise multicolor colocalization imaging.

Dichroic(s)

  • Conventional dichroic mirrors

Detectors

  • 4 High-Sensitivity Peltier-Cooled GaAsP Spectral Confocal Detectors with Olympus TruSpectral High-Efficiency Volume Phase Hologram spectral detection system (1-100 nm adjustable emission bandwidth with 2 nm spectral resolution)
  • Transmitted light detector for Brightfield/DIC Image Acquisition

Computer and Software

  • HP Windows 7 64bit Confocal Workstation and 30” Monitor
  • Complete Fluoview Acquisition & Analysis Software provides FRAP, FRET, Ratiometric Imaging Display & Analysis, 3D Image Acquisition & Display, Live 16 Channel Spectral Unmixing, Fluorescence Intensity Measurements, Time-Lapse Imaging, and More…
  • Olympus cellSens Dimension provided for additional post-acquisition data analysis and processing
  • Includes Olympus cellSens Count & Measure Solution for threshold-based object detection as well as automatic object measurement and classification

Includes Olympus cellSens 3D Deconvolution Solution utilizing Constrained Iterative Deconvolution algorithm for improved resolution, contrast and dynamic range.


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