Olympus FV4000 Confocal Laser Scanning Microscope

• Imaging diverse fluorophores in fixed or live samples
• Temperature, humidity, CO₂ control
• Spectrally tunable detectors
• Ultra-fast resonant scanning (reduced photo-damage with live samples)
• 3D z-stacks, timelapse, multi-position timelapse
• FRAP/FLIP/Photoactivation, FRET (ratio or acceptor photobleaching)

Internal user $32/hour

Module 1: Basic Introduction, Layout, Dye, and Detector Selection

Module 2: Selecting Objective

Module 3:  Imaging Parameters

Module 4: Image Acquisition, Z and Time Series, Data Saving

Module 5:  Basic Image Processing

Microscope

• Olympus IX83 fully motorized inverted microscope with laser-based autofocus (zero drift compensator)
• Automated stage with multi-area time lapse, mosaic imaging, and well-plate navigation
• Tokai hit stage-top incubation chamber (heat, humidity, CO2) for live cell imaging
• Analog and digital in-out box for synchronization with external devices

Tandem Scanners

• Galvanometer scanner for high-definition imaging (64×64 to 4096×4096 pixels, 1-1000 μs/pixel)
• Resonant scanner for high-speed imaging (1024 x 1024 pixels, 0.067 μs/pixel)

Light Sources

• Diode Lasers (wavelength in nm): 405, 445, 488, 514, 561, 594, 640, 685, 730, 785
• LED-based transmitted and epifluorescence illumination

Objectives

Air objectives:

• UPLXAPO4X, OFN 26.5, NA 0.16, WD 13 mm
• UPLXAPO10X, OFN 26.5, NA0.40, WD 3.1 mm
• UPLXAPO20X, OFN 26.5, NA 0.80, WD 0.6 mm

Silicone oil objectives:

• UPLSAPO30XS, NA 1.05, WD 0.8mm, w/CC (with correction collar)
• UPLSAPO40XS, NA 1.25, WD 0.3mm, w/CC

Immersion oil objectives:

• PL APO 60X TIRF, OFN22, NA 1.50, WD 0.11mm, w/CC

Dichroics

• Conventional dichroic mirrors

Detectors

• 6 SilVIR detectors (cooled SiPM, 2 broad-band and 4 red-shifted) with tunable emission filters via TruSpectral Volume Phase Hologram (1-100 nm adjustable emission bandwidth with 2 nm spectral resolution)
• Transmitted light detector for Brightfield/DIC imaging

Computer and Software

• HP Z8G5 with Windows 11
• Dell 43” UHD 4K Monitor
• Complete Fluoview Acquisition & Analysis Software provides FRAP, FRET, Ratiometric Imaging Display & Analysis, 3D Image Acquisition & Display, Live 16 Channel Spectral Unmixing, Fluorescence Intensity Measurements, Time-Lapse Imaging, and more…
• Olympus cellSens Dimension provided for additional post-acquisition data analysis and processing
• Includes Olympus cellSens Count & Measure Solution for threshold-based object detection as well as automatic object measurement and classification

Includes Olympus cellSens 3D Deconvolution Solution utilizing Constrained Iterative Deconvolution algorithm for improved resolution, contrast and dynamic range.