Olympus VS200 Research Slide Scanner

• High -throughput imaging of fixed samples labeled with diverse stains and fluorophores mounted on slides
• Automated image stitching, 3D z-stacks for thick slide preparations
• Automatic specimen recognition
• Semi-automated imaging of up to 210 slides at a time

Internal user $18/hour

Module 1: Introduction and Bright Field Imaging

Module 2: Fluorescence Imaging

Microscope

• Olympus VS200 research slide scanner configured for brightfield, fluorescence, darkfield and polarized light imaging, with 210 automated slide loader

Light Source

• X-Cite XYLIS II, XT730L

Dichroics

• DAPI LED Filter Set 378/52X/BS409/447/60M
• FITC LED Filter Set 474/27X/BS495/525/45M
• TRITC LED Filter Set 554/23X/BS573/609/54M
• MCherry LED Filter Set 578/21X/BS596/641/75M
• Cy5 LED Filter Set 635/18X/BS652/680/42M
• Cy7 LED Filter Set 735/28X/BS757/809/81M

Objectives

Air objectives

• 2x (NA 0.08)
• 4x (NA 0.16)
• 10x (NA 0.40)
• 20x (NA 0.80)
• 40x (NA 0.95)

Detectors

• ORCA-Fusion Gen-III sCMOS CAMERA

Software

• VS-200 ASW-FL